Anti-PD-1 [29F.1A12]

SKU: orb2645605

Description

Anti-PD-1 [29F.1A12]

Research Area

Cancer Biology

Images & Validation

• Tested Applications: Blocking, FC, IHC, WB
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 29F.1A12
• Immunogen: The antibody was raised by immunizing rats with plasmid DNA containing PD-1.
• Target: PD-1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD279; Programmed cell death protein 1; Protein PD-1; mPD-1

Flow-cytometry using the anti-PD-1 antibody 29F.1A12. Mouse splenocytes were stained with an isotype control (orb256390, black line) or the rabbit-chimeric version of 29F.1A12 (orb2645605, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-PD-1 29F.1A12. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes stained with the chimeric rabbit IgG version of 29F.1A12 (orb2645605) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645605, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-PD-1 antibody 29F.1A12. Mouse thymus (1 µg/ml) lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of X77 (orb2645605) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

PD-1

UniProt

Q02242