PC4 Antibody

• Catalog Number: orb341236
• Category: Antibodies
• Description: The PC4 Antibody is suitable for IF, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PC4 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human PC4
• UniProt ID: P11031, P53999, Q63396
• Tested applications: IF, IP, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PC4; RPO2TC1; Activated RNA polymerase II transcri
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 20024, 192269, 10923

Western blot analysis of PC4 expression in HL60 (A), Jurkat (B), mouse brain (C) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 17 kD)

Immunofluorescent analysis of PC4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.