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Anti-Paxillin (LD4 Motif) [LD4 sAB]

SKU: orb613837

Description

Rabbit monoclonal antibody to Paxillin (LD4 Motif)

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeMouse IgG
Clone No.LD4 sAB
ImmunogenPeptide corresponding to human paxillin-LD4 motif (residues 260-278) fused to MBP; peptide sequence: WGGSATRELDELMASLSD.
TargetPaxillin (LD4 Motif)
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PXN

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Anti-Paxillin (LD4 Motif) [LD4 sAB]

Immunofluorescence staining of fixed HeLa cells with anti-Paxillin (LD4 Motif) antibody LD4 sAB. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of LD4 sAB (orb613836) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nuclear periphery and diffuse cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613836, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Anti-Paxillin (LD4 Motif) [LD4 sAB] (orb613837)

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0.2 mg
$ 600.00
DispatchUsually dispatched within 5-10 working days
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