PAK4 (Phospho-S474) Antibody

• Catalog Number: orb214975
• Category: Antibodies
• Description: The PAK4 (Phospho-S474) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S474 of human PAK4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S474 of human PAK4 protein. The exact sequence is proprietary.
• UniProt ID: O96013, Q8BTW9
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA1142; Serine/threonine-protein kinase PAK 4; p
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 10298, 70584
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PAK4 (Phospho-S474) expression in PC3 (A), MCF7 (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 72 kD)

Immunohistochemical analysis of PAK4 (Phospho-S474) staining in human prostate carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK4 (Phospho-S474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).