Anti-p53 (Phospho-S6) Antibody

• Catalog Number: orb1474462
• Category: Antibodies
• Description: Rabbit monoclonal antibody to p53 (phospho-S6)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S6 of human p53 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: TP53
• Entrez: 7157
• UniProt ID: P04637
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: P53; Cellular tumor antigen p53; Antigen NY-CO-13;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p53 (Phospho-S6) expression in A549 (A), MCF7 (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 53 kD)

Immunohistochemical analysis of p53 (Phospho-S6) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.126). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.