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Anti-p53 (Phospho-S20) Antibody

Catalog Number: orb315733

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb315733
CategoryAntibodies
DescriptionRabbit polyclonal antibody to p53 (Phospho-S20)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Porcine, Primate, Rabbit
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S20 of human p53 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetTP53
Entrez7157
UniProt IDP04637
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti P53 antibody, anti Cellular tumor antigen p53
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-p53 (Phospho-S20) Antibody

Western blot analysis of p53 (Phospho-S20) expression in HCT116 (A), COS7 (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53 kD)

Anti-p53 (Phospho-S20) Antibody

Immunohistochemical analysis of p53 (Phospho-S20) staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-p53 (Phospho-S20) Antibody

Immunofluorescent analysis of p53 (Phospho-S20) staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.