P4HA3 Antibody

SKU: orb667584

Description

The P4HA3 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human P4HA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human P4HA3. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Prolyl 4-hydroxylase subunit alpha-3; 4-PH alpha-3; Procollagen-proline 2-oxoglutarate-4-dioxygenase subunit alpha-3

Western blot analysis of P4HA3 expression in HEK293T (A) whole cell lysates. (Predicted band size: 28; 61; 67 kD; Observed band size: 70 kD)

Immunofluorescent analysis of P4HA3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q6W3F0, Q6W3E9, Q7Z4N8

NCBI Gene ID

361612, 283208