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Anti-P4HA3 Antibody

Catalog Number: orb667584

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb667584
CategoryAntibodies
DescriptionRabbit polyclonal antibody to P4HA3
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant fusion protein of human P4HA3. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetP4HA3
Entrez283208, 361612
UniProt IDQ7Z4N8, Q6W3F0, Q6W3E9
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-Prolyl 4-hydroxylase subunit alpha-3 antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-P4HA3 Antibody

Western blot analysis of P4HA3 expression in HEK293T (A) whole cell lysates. (Predicted band size: 28; 61; 67 kD; Observed band size: 70 kD)

Anti-P4HA3 Antibody

Immunofluorescent analysis of P4HA3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • P4HA3 Antibody [orb352277]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • P4HA3 Antibody, HRP conjugated [orb352278]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    50 μg, 100 μg
  • P4HA3 Antibody, FITC conjugated [orb352279]

    Human

    Rabbit

    Polyclonal

    FITC

    50 μg, 100 μg
  • P4HA3 Antibody, Biotin conjugated [orb352280]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    100 μg, 50 μg