Anti-p27 Kip1 (Phospho-T198) Antibody

• Catalog Number: orb213715
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CDKN1B
• Target: CDKN1B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T198 of human p27 Kip1 protein. The exact sequence is proprietary.
• UniProt ID: P46414, P46527
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti KIP1 antibody, anti Cyclin-dependent kinase i
• Note: For research use only
• Entrez: 12576, 1027

Western blot analysis of p27 Kip1 (Phospho-T198) expression in HEK293T (A), Hela (B), rat lung (C), rat kidney (D) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of p27 Kip1 (Phospho-T198) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.