Anti-p27 KIP 1/CDKN1B Antibody

• Catalog Number: orb196241
• Category: Antibodies
• Description: Anti-p27 KIP 1/CDKN1B Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.
• UniProt ID: P46527
• MW: 27 kDa
• Tested applications: FC, ICC, IF, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cyclin-dependent kinase inhibitor 1B; Cyclin-depen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of p27 KIP 1/CDKN1B using anti-p27 KIP 1/CDKN1B antibody.Lane 1:human HeLa cell;2:human MCF-7 cell.

IF analysis of p27 KIP 1/CDKN1B using anti-p27 KIP 1/CDKN1B antibody. p27 KIP 1/CDKN1B was detected in an immunocytochemical section of U20S cells.