Anti-P-glycoprotein [MRK16]

SKU: orb348896

Description

Rabbit monoclonal antibody to P-glycoprotein

Images & Validation

• Tested Applications: FC, IF, IP
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: MRK16
• Immunogen: Drug resistant K-562 cells.
• Target: P-glycoprotein
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ABCB1; Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; CD243; MDR1; PGP; PGY1

Flow-cytometry using the anti-P-glycoprotein antibody MRK16 HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (orb348896, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

P-glycoprotein

UniProt

P08183