Anti-P-glycoprotein [MRK16]

• Catalog Number: orb348896
• Category: Antibodies
• Description: Rabbit monoclonal antibody to P-glycoprotein
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MRK16
• Tested applications: FC, IF, IP
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Drug resistant K-562 cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: P-glycoprotein
• UniProt ID: P08183
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ABCB1; Multidrug resistance protein 1; ATP-binding
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-P-glycoprotein antibody MRK16 HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (orb348896, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.