Anti-OTUB1 Antibody

• Catalog Number: orb515291
• Category: Antibodies
• Description: Rabbit polyclonal antibody to OTUB1
• Target: OTUB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human OTUB1. The exact sequence is proprietary.
• UniProt ID: B2RYG6, Q96FW1, Q7TQI3
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: OTB1; OTU1; Ubiquitin thioesterase OTUB1; Deubiqui
• Note: For research use only
• Entrez: 293705, 55611, 107260

Western blot analysis of OTUB1 expression in HEK293T (A), Jurkat (B), NIH3T3 (C), mouse brain (D), H9C2 (E), PC12 (F) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 35; 31 kD)

Immunohistochemical analysis of OTUB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.