Oncostatin M/Osm Rabbit Polyclonal Antibody

SKU: orb402405

Description

Anti-Oncostatin M/Osm Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Research Area

Epigenetics & Chromatin, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml ELISA (Cap), 1-5μg/ml
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse Oncostatin M recombinant protein (Position: N25-R206).
• Target: Oncostatin-M
• Molecular Weight: 28 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Oncostatin-M; OSM; Osm

Sandwich ELISA - Recombinant mouse Oncostatin M/Osm protein standard curve. Use in combination with reagents from Mouse Oncostatin M/Osm ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of Oncostatin M using anti-Oncostatin M antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates, Lane 2: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 28KD. The expected band size for Oncostatin M is at 28KD.

Quick Database Links

Gene Symbol

Oncostatin-M

UniProt

P53347