Anti-Nucleophosmin Antibody

• Catalog Number: orb1474063
• Category: Antibodies
• Description: Mouse monoclonal antibody to Nucleophosmin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human Nucleophosmin. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NPM1
• Entrez: 4869
• UniProt ID: P06748
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Nucleophosmin expression in Hela (A), Jurkat (B), MCF7 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 38 kD)

Immunohistochemical analysis of Nucleophosmin staining in human thymus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.