NTB-A Antibody

• Catalog Number: orb1174010
• Category: Antibodies
• Description: Anti-NTB-A antibody(DM159), Rabbit mAb
• Target: NTB-A
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q96DU3
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM159
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NTB-A;SLAMF6;Ly108;NK-T-B-antigen;CD352;KALI
• Note: For research use only

Flow cytometry analysis with Anti-NTB-A on Expi293 cells transfected with human NTB-A (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb (orb1174010). (A) orb1174010 does not bind to 293T cells that do not express NTB-A. (B) A clear peak shift of orb1174010 was seen compared to the control when incubated with NTB-A-expressing Raji cells, indicating strong binding of orb1174010 to NTB-A. Antibodies were incubated at 5 μg/mL.

Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb (orb1174010). (A) orb1174010 does not bind to 293T cells that do not express NTB-A. (B) A clear peak shift of orb1174010 was seen compared to the control when incubated with NTB-A-expressing Jurkat cells, indicating strong binding of orb1174010 to NTB-A. Antibodies were incubated at 5 μg/mL.

Anti-NTB-A antibody (SKU# orb1174010) at 1/1000 dilution.Lane: RAJI (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 37 kDa.Observed band size: 60 kDa

orb1174010 at 10 μg/ml staining NTB-A in Raji cells by IHC (SKU# orb1174010)