Anti-Notch 2 [B9]

• Catalog Number: orb411557
• Category: Antibodies
• Description: Human monoclonal antibody to Notch 2
• Target: Notch 2
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: NRR of mouse Notch 2.
• UniProt ID: O35516, Q04721
• Tested applications: Blocking, ELISA, IF
• Clone Number: B9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AGS2; NOTCH2; HJCYS; hN2
• Research Area: Molecular Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (orb411558) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb411558, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.