Anti-Notch 1 [E6]

SKU: orb411554

Description

Human monoclonal antibody to Notch 1

Research Area

Molecular Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human, Mouse

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Human IgG
• Clone No.: E6
• Immunogen: This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to the NRR of mouse Notch1.
• Target: Notch 1
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tan1; Notch1; Mis6; N1; lin-12; NOTCH1; TAN1; hN1

Flow-cytometry using the anti-Notch 1 antibody E6 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (orb411554, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of E6 (orb411554) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb411554, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Notch 1

UniProt

P46531, Q01705