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Anti-NNMT Antibody

Catalog Number: orb341145

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb341145
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NNMT
TargetNNMT
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenRecombinant full length protein of human NNMT
UniProt IDO55239, P40261
Tested applicationsIF, IH, WB
Dilution rangeWB: 1:500-1:2000, IF/ICC: 1:50-1:200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNicotinamide N-methyltransferase
NoteFor research use only
Entrez4837, 18113
Anti-NNMT Antibody

Western blot analysis of NNMT expression in Hela (A), HepG2 (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 28 kD)

Anti-NNMT Antibody

Immunohistochemical analysis of NNMT staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NNMT Antibody

Immunofluorescent analysis of NNMT staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    ELISA,  WB

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    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

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