Anti-NMDAR2B (Phospho-Y1474) Antibody

• Catalog Number: orb393306
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GRIN2B (Phospho-Y1474)
• Target: GRIN2B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1474 of human NMDAR2B protein. The exact sequence is proprietary.
• UniProt ID: Q01097, Q13224, Q00960
• Tested applications: IH, WB
• Dilution range: WB: 1:500:1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Glutamate [NMDA] receptor subunit epsilon-2 a
• Note: For research use only
• Entrez: 14812, 24410, 2904

Western blot analysis of NMDAR2B (Phospho-Y1474) expression in HEK293T UV-treated (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 166 kD; Observed band size: 190 kD)

Immunohistochemical analysis of NMDAR2B (Phospho-Y1474) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.