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Anti-NMDAR1 Antibody

Catalog Number: orb665700

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb665700
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NMDAR1
TargetGRIN1
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
PurificationThe antibody was purified by immunogen affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NMDAR1. The exact sequence is proprietary.
UniProt IDP35439, P35438, Q05586
Tested applicationsIF, IH, WB
Dilution rangeWB: 1-500-1000, IHC-P: 1-50-200, IF: 1-100-500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-NMDAR1 antibody, anti-Glutamate receptor iono
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NoteFor research use only
Entrez2902, 24408, 14810
Anti-NMDAR1 Antibody

Western blot analysis of NMDAR1 expression in C6 (A), MCF7 (B), U87MG (C), Myla2059 (D) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 130 kD)

Anti-NMDAR1 Antibody

Immunohistochemical analysis of NMDAR1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NMDAR1 Antibody

Immunofluorescent analysis of NMDAR1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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