NMDA NR2B Subunit (Tyr1252) Antibody

SKU: orb319605

Description

Our Anti-NMDA NR2B Subunit (Tyr1252) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1252) and is antigen affinity purified from pooled serum.

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.736111111, ICC: 1:100-1:400
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Primate, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
• Target: NMDA NR2B Subunit (Tyr1252)
• Molecular Weight: 180
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 (red, 1:400) and PSD95 (green).

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1252 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Quick Database Links

Gene Symbol

NMDA NR2B Subunit (Tyr1252)

UniProt

Q00960