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Anti-NHERF1 Purified
Catalog Number: orb43765
| Catalog Number | orb43765 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to NHERF1. |
| Clonality | Monoclonal |
| Clone Number | EBP-10 |
| Tested applications | IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Bacterially produced recombinant full-length human NHERF1. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | NHERF1 |
| Entrez | 9368 |
| UniProt ID | O14745 |
| RRID | AB_10996959 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | NHERF1 antibody, anti-EBP50 Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 2 μg/ml; 60 min on vertical incubator, positive control: RAJI human lymphoma cell lysate, non-reducing conditions, 10% separating SDS-PAGE gel. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.
