Anti-NF-kappaB p65 (Phospho-S529) Antibody

• Catalog Number: orb223997
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NFkB p65 (phospho-S529)
• Target: RELA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Primate
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S529 of human NF-kappaB p65 protein. The exact sequence is proprietary.
• UniProt ID: Q04206
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NF kappa B p65delta3 antibody, anti NFKB 3 an
• Note: For research use only
• Entrez: 5970

Western blot analysis of NF-kappaB p65 (Phospho-S529) expression in MCF7 (A), MCF7 TNFa treated 5 min (B), MCF7 TNFa treated 30 min (C), HEK293T (D), Hela (E) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 75 kD)

Immunohistochemical analysis of NF-kappaB p65 (Phospho-S529) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.