Neurofilament NF-L Antibody

SKU: orb319587

Description

Our Anti-Neurofilament NF-L primary antibody from Biorbyt is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is total IgY fraction.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1:10000, IHC: 1.430555556, ICC: 1:2000-1:5000
• Reactivity: Bovine, Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Preparation of bovine spinal cord NF-L.
• Target: Neurofilament NF-L
• Molecular Weight: 68
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 21.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

68 kDa neurofilament protein antibody, 68kDa Neurofilament antibody, 68kDa neurofilament protein antibody, CMT1F antibody, CMT2E antibody, FLJ53642 antibody, Light molecular weight neurofilament protein antibody, NEFL antibody, Neurofilament light antibody, Neurofilament light polypeptide 68kDa antibody, Neurofilament light polypeptide antibody, Neurofilament protein, light chain antibody, Neurofilament subunit NF L antibody, Neurofilament triplet L protein antibody, NF-L antibody, NF68 antibody, NFL antibody, NFL_HUMAN antibody

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (Cat. orb319587, 1:2000, green) and labeling of FOX3/NeuN (Cat. orb613671, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody (Cat. orb319587, red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Quick Database Links

Gene Symbol

Neurofilament NF-L

UniProt

P02548