Neurofilament NF-L Antibody

SKU: orb319586

Description

Our Anti-Neurofilament NF-L primary antibody from Biorbyt is mouse monoclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is Protein G purified.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:100-1:1000, ICC: 1:100-1:1000
• Reactivity: Bovine, Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: DA2
• Immunogen: Preparation of porcine NF-L.
• Target: Neurofilament NF-L
• Molecular Weight: 68
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 2 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

68 kDa neurofilament protein antibody, 68kDa Neurofilament antibody, 68kDa neurofilament protein antibody, CMT1F antibody, CMT2E antibody, FLJ53642 antibody, Light molecular weight neurofilament protein antibody, NEFL antibody, Neurofilament light antibody, Neurofilament light polypeptide 68kDa antibody, Neurofilament light polypeptide antibody, Neurofilament protein, light chain antibody, Neurofilament subunit NF L antibody, Neurofilament triplet L protein antibody, NF-L antibody, NF68 antibody, NFL antibody, NFL_HUMAN antibody

Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (Cat. orb319586, 1:500, red) and labeling of GFAP (Cat. orb319550, 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (Cat. orb319585, red, 1:25,000) and anti-NFL antibody (Cat. orb319586, green, 1:100).

Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (Cat. orb319586, green, 1:100) and anti-alpha internexin.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Quick Database Links

Gene Symbol

Neurofilament NF-L

UniProt

P02547