Anti-Nerve growth factor [mab 911]

SKU: orb1821525

Description

Anti-Nerve growth factor [mab 911]

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: Blocking, Crystallography, ELISA, In vitro, In vivo, NeA
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: mab 911
• Target: Nerve growth factor
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beta-nerve growth factor; Beta-NGF

Flow cytometry using anti-Nerve growth factor antibody mab 911. Paraformaldehyde fixed NIH3T3 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of mab 911 (orb1821525, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of NIH3T3 cells with anti-Nerve growth factor antibody mab 911. Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of mab 911 (orb1821525) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821525, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Nerve growth factor antibody mab 911. Human brain cerebellum tissue lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the monoclonal antibody mab 911 (orb1821525) at 1 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

Nerve growth factor

UniProt

P01138