NEXN Antibody

SKU: orb667434

Description

Rabbit polyclonal antibody to Nelin

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human Nelin. The exact sequence is proprietary.
• Target: NEXN
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Anti-Nexilin antibody, anti-F-actin-binding protein antibody, anti-Nelin antibody

Western blot analysis of Nelin expression in SGC7901 (A), mouse testis (B), rat brain (C), rat spleen (D) whole cell lysates. (Predicted band size: 72; 73; 79; 80 kD; Observed band size: 81 kD)

Immunofluorescent analysis of Nelin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

NEXN

UniProt

Q9Z2J4, Q0ZGT2, Q7TPW1

NCBI Gene ID

91624, 246172, 68810