Anti-NEFH Antibody

• Catalog Number: orb412733
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NEFH
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human NEFH
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NEFH
• Entrez: 380684, 4744
• UniProt ID: P16884, P19246, P12036
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-KIAA0845 antibody, anti-NFH antibody, anti-Ne
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NEFH expression in mouse brain (A) whole cell lysates. (Predicted band size: 105; 112 kD; Observed band size: 150; 240 kD)

Immunofluorescent analysis of NEFH staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).