NEFH Mouse Monoclonal Antibody

SKU: orb18247

Description

NEFH Mouse Monoclonal Antibody

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, - Western blot, 0.5ml, Human, mouse, rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: N52
• Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
• Target: Neurofilament heavy polypeptide
• Molecular Weight: 200 kDa
• Purification: Ascites
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; Nefh; Nfh

Immunohistochemical analysis of paraffin-embedded rat brain tissue using NEFH antibody

Western blot analysis of rat brain tissue lysates (Lane1), mouse brain tissue lysates (Lane2) using NEFH antibody

Quick Database Links

Gene Symbol

Neurofilament heavy polypeptide

UniProt

P16884

Lingyi Kong 1, Heidi Olze 1, Agnieszka J Szczepek 2 3 Histamine H3 Receptor Antagonists Influence the Directional Growth of Type II Spiral Ganglion Neurites Within the Developing Cochlea of C57BL/6 Mice Neurochem Res, (2025)

PubMed: 40801998

Applications

IF

Reactivity

Mouse

Zhao, Quanlai et al. Cytoprotective effects of myristicin against hypoxia‑induced apoptosis and endoplasmic reticulum stress in rat dorsal root ganglion neurons Mol Med Rep, 15, 2280-2288 (2017)

PubMed: 28260107