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Anti-Nectin-4(enfortumab biosimilar) mAb

Catalog Number: orb1291227

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1291227
CategoryAntibodies
DescriptionAnti-Nectin-4(enfortumab biosimilar) mAb
Species/HostHuman
ClonalityMonoclonal
Tested applicationsELISA, FC
ReactivityHuman
IsotypeIgG1
Antibody TypeBiosimilar Antibody
Dilution rangeELISA 1:5000-10000|Flow Cyt 1:100
Form/AppearancePowder
ConjugationUnconjugated
TargetNECTIN4
UniProt IDQ96NY8
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesEDSS1;LNIR;nectin-4;PRR4;PVRL4
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NoteFor research use only
Application notesLyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date12 months from date of receipt.
Anti-Nectin-4(enfortumab biosimilar) mAb

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag orb1173741 can bind Anti-Nectin-4 Neutralizing antibody (orb1291227) in a linear range of 0.61–39.06 ng/ml.

Anti-Nectin-4(enfortumab biosimilar) mAb

Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (orb1291227) on Expi293 cells transfected with Human Nectin-4 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Anti-Nectin-4(enfortumab biosimilar) mAb

Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb (orb1291227). (A) orb1291227 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of orb1291227 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of orb1291227 to Nectin-4. Antibodies were incubated at 2 μg/mL.