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Item 1 of 3
NECTIN4 Biosimilar Antibody
Catalog Number: orb1291227
| Catalog Number | orb1291227 |
|---|---|
| Category | Antibodies |
| Description | Anti-Nectin-4(enfortumab biosimilar) mAb |
| Target | NECTIN4 |
| Clonality | Monoclonal |
| Species/Host | Human |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Powder |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | Q96NY8 |
| Tested applications | ELISA, FC |
| Dilution range | ELISA 1:5000-10000 Flow Cyt 1:100 |
| Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Antibody Type | Biosimilar Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | EDSS1LNIRnectin-4PRR4PVRL4 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag orb1173741 can bind Anti-Nectin-4 Neutralizing antibody (orb1291227) in a linear range of 0.61–39.06 ng/ml.
Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (orb1291227) on Expi293 cells transfected with Human Nectin-4 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb (orb1291227). (A) orb1291227 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of orb1291227 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of orb1291227 to Nectin-4. Antibodies were incubated at 2 μg/mL.
