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Catalog Number | orb1291227 |
---|---|
Category | Antibodies |
Description | Anti-Nectin-4(enfortumab biosimilar) mAb |
Species/Host | Human |
Clonality | Monoclonal |
Tested applications | ELISA, FC |
Reactivity | Human |
Isotype | IgG1 |
Antibody Type | Biosimilar Antibody |
Dilution range | ELISA 1:5000-10000|Flow Cyt 1:100 |
Form/Appearance | Powder |
Conjugation | Unconjugated |
Target | NECTIN4 |
UniProt ID | Q96NY8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | EDSS1;LNIR;nectin-4;PRR4;PVRL4 Read more... |
Note | For research use only |
Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Expiration Date | 12 months from date of receipt. |
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag orb1173741 can bind Anti-Nectin-4 Neutralizing antibody (orb1291227) in a linear range of 0.61–39.06 ng/ml.
Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (orb1291227) on Expi293 cells transfected with Human Nectin-4 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb (orb1291227). (A) orb1291227 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of orb1291227 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of orb1291227 to Nectin-4. Antibodies were incubated at 2 μg/mL.
ELISA, FC | |
Human | |
Human | |
Monoclonal | |
Unconjugated |
FC | |
Human | |
Human | |
Monoclonal | |
Unconjugated |