NCC (Thiazide sensitive NaCl cotransporter) Antibody

SKU: orb55909

Description

Our Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum.

Research Area

Cell Biology, Kidney & Urological Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 1:1000-1:6000, IHC: 1:100-1:10,000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Guinea pig, Hamster

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).
• Target: NCC (Thiazide sensitive NaCl cotransporter)
• Molecular Weight: 160
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody

Immunolabeling of mouse kidney paraffin section specifically detecting NCC Thr53 (Cat. orb55909, red, 1:1000) phosphorylation with the increased NCC activation in Ang-II-infused Prdx5 KO kidney.

Immunostaining of mouse kidney sections from control (CTL) and BRL37344 treated (BRL, 1 mg/kg) specifically labeling localized phosphorylated NCC thr53 (Cat. orb55909, green) and counterstained with Evans Blue (in red).

Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (Cat. orb55909, red, 1:100,000) on the left and the absence of staining in the KO on the right.

Two Western blots show the abundance of pNCCT53 (Cat. orb55909, 1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1–KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW).

Western blot image of kidney measuring levels of pNCC-T53 and total NCC in Kir4.2+/+ and Kir4.2−/− mice.

Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Quick Database Links

Gene Symbol

NCC (Thiazide sensitive NaCl cotransporter)

UniProt

P59158