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Anti-N-WASP Antibody

Catalog Number: orb382119

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb382119
CategoryAntibodies
DescriptionRabbit polyclonal antibody to WASL.
TargetWASL
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenRecombinant full length protein of human N-WASP
UniProt IDO08816, O00401, Q91YD9
Tested applicationsIF, IH, WB
Dilution rangeWB: 1:500-2000, IF/ICC: 1:50-200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNeural Wiskott-Aldrich syndrome protein; N-WASP
NoteFor research use only
Entrez8976, 73178
Anti-N-WASP Antibody

Western blot analysis of N-WASP expression in SW480 (A), MCF7 (B), mouse brain (C), mouse kidney (D) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 55 kD)

Anti-N-WASP Antibody

Immunohistochemical analysis of N-WASP staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-N-WASP Antibody

Immunofluorescent analysis of N-WASP staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Anti-N WASP/WASL Antibody [orb670331]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
  • WASL Antibody [orb682250]

    ELISA,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Anti-N WASP/WASL Antibody [orb2603782]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-N WASP/WASL Antibody [orb2603783]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-N WASP/WASL Antibody [orb2603784]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg