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Item 1 of 2
Anti-N-SMase3 Antibody
Catalog Number: orb668202
| Catalog Number | orb668202 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to N-SMase3 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse |
| Immunogen | Recombinant fusion protein of human N-SMase3. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1-500-2000 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | SMPD4 |
| Entrez | 55627, 77626 |
| UniProt ID | Q9NXE4, Q6ZPR5 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | Anti-KIAA1418 antibody, anti-Sphingomyelin phospho Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of N-SMase3 expression in A549 (A) whole cell lysates. (Predicted band size: 44; 56-69; 83-98 kD; Observed band size: 100 kD)
Immunofluorescent analysis of N-SMase3 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
