Myosin Light Chain 2 Rabbit Monoclonal Antibody

SKU: orb548173

Description

Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 IP 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: FHO-13
• Immunogen: A synthesized peptide derived from human Myosin Light Chain 2
• Target: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Molecular Weight: 29 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; Cardiac myosin light chain 2; Myosin light chain 2, slow skeletal/ventricular muscle isoform; MLC-2s/v; Ventricular myosin light chain 2; MYL2; MLC2

Western blot analysis of Myosin Light Chain 2 expression in Mouse heart lysate (orb548173). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL2 monoclonal antibody (Catalog # orb548173) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for MYL2.

Quick Database Links

Gene Symbol

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

UniProt

P10916