Anti-Myelin basic protein [F28C4]

• Catalog Number: orb2645601
• Category: Antibodies
• Description: Anti-Myelin basic protein [F28C4]
• Target: Myelin basic protein
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing PL/J mice with MBP acetyl residues 1-9.
• UniProt ID: P02686
• Tested applications: ELISA
• Clone Number: F28C4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MBP; Myelin A1 protein; Myelin membrane encephalit
• Note: For research use only

Immunofluorescence staining of Kelly cells with anti-Myelin basic protein antibody F28C4. Immunofluorescence analysis of paraformaldehyde-fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of F28C4 (orb2645601) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645601, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Myelin basic protein antibody F28C4. Human brain cerebellum (A) (0.0001 µg/ml) and hippocampus (B) (5 µg/ml) tissue lysates (5 µg protein in RIPA buffer) and cerebral cortex (C) (0.001 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of F28C4 (orb2645601) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.