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Item 1 of 3
Anti-MVB12A Antibody
Catalog Number: orb1879137
| Catalog Number | orb1879137 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to MVB12A |
| Clonality | Polyclonal |
| Tested applications | FC, IF, WB |
| Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human MVB12A. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/30) |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Entrez | 93343 |
| UniProt ID | Q96EY5 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CFBP; FAM125A; Multivesicular body subunit 12A; CI Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of MVB12A expression in PC3 (A) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 29 kD)
Immunofluorescent analysis of Anti-MVB12A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of A431 cells using Anti-MVB12A Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
