Munc-18 (Ser 513) Antibody

SKU: orb319581

Description

Our Anti-Munc-18 (Ser 513) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects rat Munc-18 (Ser 513) and is antigen affinity purified from pooled serum.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: WB: 0.736111111, ICC: 0.319444444
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Mouse, Primate, Xenopus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH).
• Target: Munc-18 (Ser 513)
• Molecular Weight: 65
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLJ37475 antibody, Munc 18 1 antibody, Munc 18a antibody, MUNC18 1 antibody, N-Sec1 antibody, Neuronal SEC1 antibody, NSec1 antibody, p67 antibody, Protein unc-18 homolog 1 antibody, Protein unc-18 homolog A antibody, Rb sec1 antibody, RBSEC1 antibody, STXB1_HUMAN antibody, STXBP1 antibody, Syntaxin binding protein 1 antibody, Syntaxin-binding protein 1 antibody, Unc 18 homolog antibody, Unc 18A antibody, Unc-18A antibody, Unc18 1 antibody, UNC18 antibody, Unc18-1 antibody

Immunostaining of 14 DIV rat cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser513 (Cat. orb319581, 1:400,m red) and PSD95 (green).

Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser513 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Quick Database Links

Gene Symbol

Munc-18 (Ser 513)

UniProt

Q62753