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Anti-MUC1 (Phospho-Y1229) Antibody

Catalog Number: orb214279

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb214279
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MUC1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1229 of human MUC1 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMUC1
Entrez4582, 17829
UniProt IDP15941, Q02496
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti PUM antibody, anti Mucin-1 antibody, anti MUC
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MUC1 (Phospho-Y1229) Antibody

Western blot analysis of MUC1 (Phospho-Y1229) expression in MCF7 PMA-treated (A), BT20 (B), NIH3T3 UV-treated (C), H9C2 PMA-treated (D) whole cell lysates. (Predicted band size: 122 kD; Observed band size: 170 kD)

Anti-MUC1 (Phospho-Y1229) Antibody

Immunohistochemical analysis of MUC1 (Phospho-Y1229) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MUC1 (Phospho-Y1229) Antibody

Immunofluorescent analysis of MUC1 (Phospho-Y1229) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.