Anti-MUC1 [PAM4]

• Catalog Number: orb1152459
• Category: Antibodies
• Description: Mouse monoclonal antibody to MUC1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAM4
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was raised by immunization of mice with the PAM4 antigen.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: MUC1
• UniProt ID: P15941
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Mucin-1; Breast carcinoma-associated antigen DF3;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-MUC1 antibody PAM4. Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of PAM4 (orb1152458, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-MUC1 PAM4. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of PAM4 (orb1152458) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152458, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.