mTOR Antibody

SKU: orb213950

Description

The mTOR Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mTOR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mTOR. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1

Western blot analysis of mTOR expression in HepG2 (A), MCF7 (B), H9C2 (C), AML12 (D) whole cell lysates. (Predicted band size: 288 kD; Observed band size: 289 kD)

Immunohistochemical analysis of mTOR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of mTOR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q9JLN9, P42346, P42345

NCBI Gene ID

2475, 56718, 56717

References

Nan Huang 1, Junxi Ren 1, Xinyue Deng 1, Qize Bao 1, Genjie Huang 1, Shimeng Zhi 1 2, Yuedan Li 1, Juan Li 1, Binghui Hu 1, Dongqiang Zeng 1, Huiying Sun 1, Wei Zeng 3, Min Shi 1, Wangjun Liao 1 3, Jianhua Wu 1, Na Huang 1 Endothelial F3-mediated autolysosome and lipid metabolism promote resistance to anti-VEGFA therapy in metastatic colorectal cancer Autophagy ., (2025)

PubMed: 40922383

Applications

WB

Reactivity

Mouse