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Anti-MTHFD2 Antibody

Catalog Number: orb411750

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb411750
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MTHFD2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human MTHFD2
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMTHFD2
Entrez10797, 17768
UniProt IDP18155, P13995
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-NMDMC antibody, anti-Bifunctional methylenete
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MTHFD2 Antibody

Western blot analysis of MTHFD2 expression in LO2 (A), HT29 (B), mouse pancreas (C) whole cell lysates. (Predicted band size: 26; 37 kD; Observed band size: 38 kD)

Anti-MTHFD2 Antibody

Immunohistochemical analysis of MTHFD2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-MTHFD2 Antibody

Immunofluorescent analysis of MTHFD2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • MTHFD2L Antibody [orb355751]

    ELISA,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • Anti-MTHFD2 Antibody [orb1474000]

    WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • MTHFD2L Antibody, Biotin conjugated [orb355752]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    100 μg, 50 μg
  • MTHFD2L Antibody, FITC conjugated [orb355753]

    Human

    Rabbit

    Polyclonal

    FITC

    100 μg, 50 μg
  • MTHFD2L Antibody, HRP conjugated [orb355754]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    100 μg, 50 μg