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Anti-MTA2 Monoclonal Antibody
Catalog Number: orb548157
| Catalog Number | orb548157 |
|---|---|
| Category | Antibodies |
| Description | Anti-MTA2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Immunogen | A synthesized peptide derived from human MTA2 The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation. |
| UniProt ID | O94776 |
| MW | 75 kDa |
| Tested applications | FC, ICC, IF, IHC, WB |
| Application notes | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200FC 1:100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Antibody Type | Primary Antibody |
| Clone Number | ACOE-13 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TNF receptor-associated factor 4; Cysteine-rich do Read more... |
| Note | For research use only |
Western blot analysis of MTA2 using anti-MTA2 antibody (orb548157) in HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # orb548157) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.
