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Item 1 of 2
Anti-MSK1 (Phospho-S376) Antibody
Catalog Number: orb393153
| Catalog Number | orb393153 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to RPS6KA5 (Phospho-S376) |
| Target | RPS6KA5 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S376 of human MSK1 protein. The exact sequence is proprietary. |
| UniProt ID | O75582 |
| Tested applications | IF, WB |
| Dilution range | WB: 1:500:1000, IF/ICC: 1:100:500 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alp Read more... |
| Note | For research use only |
| Entrez | 9252 |
Western blot analysis of MSK1 (Phospho-S376) expression in K562 (A), HEK293T (B) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 90 kD)
Immunofluorescent analysis of MSK1 (Phospho-S376) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
