Anti-MSH2 [1B11-14-16]

SKU: orb1821561

Description

Anti-MSH2 [1B11-14-16]

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 1B11-14-16
• Immunogen: The original antibody was generated by immunizing BALB/c mice with human MSH2 protein peptide (KLH-NSFVNEIISRIKVTT-Cys) conjugated with KLH in equal volume of Freund's complete adjuvant.
• Target: MSH2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hMSH2; DNA mismatch repair protein Msh2; MutS protein homolog 2; CGMCC NO.17405; G219-1129

Flow cytometry using anti-MSH2 antibody 1B11-14-16. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 1B11-14-16 (orb1821562, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-MSH2 antibody 1B11-14-16. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1B11-14-16 (orb1821562) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821562, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-MSH2 antibody 1B11-14-16. HEK293 (A) (0.01 µg/ml), A431 (B) (0.01 µg/ml), HeLa (C) (0.01 µg/ml), HepG2 (D) (0.03 µg/ml), K562 (E) (0.03 µg/ml) cells nuclear lysate, and A549 (F) (0.03 µg/ml) cells lysate (35 µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 1B11-14-16 (orb1821562) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

MSH2

UniProt

P43246