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Description
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| Tested Applications | FC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rat IgG2b |
| Clone No. | RB6-8C5 |
| Immunogen | Murine granulocytes |
| Target | Ly6G |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Gr1, Gr-1, Ly-6G
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Separation of murine myeloid Ly6G positive cells stained using anti-Ly6G (RB-8C5) Biotin antibody (concentration in sample 1.0 μg/ml, Streptavidin APC, red-filled) from murine myeloid cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis.
Flow cytometry surface staining pattern of murine peritoneal fluid cell suspension stained using anti-murine Ly6G (RB6-8C5) Biotin antibody (concentration in sample 1.0 μg/ml, Streptavidin APC).
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UniProt Details
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NCBI Gene Details
− No NCBI Gene data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
Ly6G Antibody (Biotin) (orb756847)
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