MMP2 Mouse Monoclonal Antibody

SKU: orb1474304

Description

The MMP2 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human MMP-2 protein expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human MMP-2 protein expressed in E.coli.
• Target: MMP2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLG4A; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1

Western blot analysis of MMP2 expression in Raw264.7 (A) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 74 kD)

Immunofluorescent analysis of MMP2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MMP2

UniProt

P33434, P08253

NCBI Gene ID

17390, 4313