Anti-MLL5 Antibody

• Catalog Number: orb412434
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KMT2E
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human MLL5
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200, IP: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KMT2E
• Entrez: 69188, 55904
• UniProt ID: Q3UG20, Q8IZD2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-MLL5 antibody, anti-Histone-lysine N-methyltr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MLL5 expression in MCF7 (A), Hela (B), mouse lung (C), mouse brain (D) whole cell lysates. (Predicted band size: 68; 98; 131; 180-204 kD; Observed band size: 100 kD)

Immunohistochemical analysis of MLL5 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MLL5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.