Anti-MHC II [P7/7]

• Catalog Number: orb758780
• Category: Antibodies
• Description: Rat monoclonal antibody to MHC II
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: P7/7
• Tested applications: FC, IHC, IP
• Reactivity: Mouse, Rat
• Isotype: IgG2b
• Immunogen: This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: MHC II
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: P7.7; I-A; Ia; I-E; MHC-II; MHC Class II
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-MHC II antibody P7/7. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of P7/7 (orb758779, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-MHC II antibody P7/7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of P7/7 (orb758779) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758779, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.