Anti-mGLUR7 Antibody

• Catalog Number: orb515214
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GRM7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human mGLUR7. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GRM7
• Entrez: 108073, 81672, 2917
• UniProt ID: P35400, Q14831, Q68ED2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: GPRC1G; MGLUR7; Metabotropic glutamate receptor 7;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mGLUR7 expression in mouse brain (A), rat brain (B), HEK293T (C), Hela (D), MCF7 (E) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 102 kD)

Immunofluorescent analysis of mGLUR7 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.