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Anti-mGluR1 GRM1 Rabbit Monoclonal Antibody

Catalog Number: orb548154

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb548154
CategoryAntibodies
DescriptionAnti-mGluR1 GRM1 Rabbit Monoclonal Antibody. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
ClonalityMonoclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
ImmunogenA synthesized peptide derived from human mGluR1
UniProt IDQ13255
MW65 kDa
Tested applicationsWB
Application notesWB 1:500-1:2000. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Antibody TypePrimary Antibody
Clone NumberIGI-7
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMetabotropic glutamate receptor 1; mGluR1; GRM1; G
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NoteFor research use only
Anti-mGluR1 GRM1 Rabbit Monoclonal Antibody

Western blot analysis of mGluR1 expression in Mouse brain lysate (orb548154). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM1 monoclonal antibody (Catalog # orb548154) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for GRM1.

Anti-mGluR1 GRM1 Rabbit Monoclonal Antibody

Western blot analysis of mGluR1 expression in Mouse brain lysate (orb548154). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM1 monoclonal antibody (Catalog # orb548154) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for GRM1.