Metadherin Antibody

SKU: orb1474245

Description

The Metadherin Antibody is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of human Metadherin expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Disclaimer: For research use only

Alternative Names

AEG1; LYRIC; Protein LYRIC; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein

Western blot analysis of Metadherin expression in Jurkat (A), A549 (B), MCF7 (C), Hela (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of Metadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.71). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Metadherin in Hela cells (green) and negative control (purple).

Quick Database Links

UniProt

Q86UE4

NCBI Gene ID

92140