Metadherin Antibody

• Catalog Number: orb1474245
• Category: Antibodies
• Description: The Metadherin Antibody is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of human Metadherin expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.
• UniProt ID: Q86UE4
• Tested applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AEG1; LYRIC; Protein LYRIC; 3D3/LYRIC; Astrocyte e
• Note: For research use only
• Entrez: 92140

Western blot analysis of Metadherin expression in Jurkat (A), A549 (B), MCF7 (C), Hela (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of Metadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.71). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Metadherin in Hela cells (green) and negative control (purple).